pC0061 PsmCas13 (B05) His6-TwinStrep-SUMO-BsaI

LOCUS       pC0061	PsmCas13 (B05) His6-TwinStrep-SUMO-BsaI               10050 bp ds-DNA     circular SYN 20-SEP-2018
DEFINITION  Expresses PsmCas13b from a SUMO-tagged bacterial expression 
            construct.
ACCESSION   .
VERSION     .
KEYWORDS    pC0061	PsmCas13 (B05) His6-TwinStrep-SUMO-BsaI
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10050)
  AUTHORS   Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins JJ, Zhang 
            F
  TITLE     Multiplexed and portable nucleic acid detection platform with Cas13,
            Cas12a, and Csm6.
  JOURNAL   Science. 2018 Apr 27;360(6387):439-444. doi: 
            10.1126/science.aaq0179. Epub 2018 Feb 15.
  PUBMED    29449508
REFERENCE   2  (bases 1 to 10050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 20, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10050
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    1..25
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             40..62
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             81..98
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             108..125
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             138..161
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             198..221
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             222..512
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIG"
     primer_bind     complement(4532..4550)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4546..4593
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(4768..4796)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(4823..4841)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4909..5013
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5014..5874
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5232..5251)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6045..6633
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6534..6553
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6787..6804
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6819..6958
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6884..6903)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7044..7066
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7060..7251)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(8563..9645)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     9607..9626
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9646..9723)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     9929..9948
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     10032..1
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10032..10050
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact ttaagaagga
       61 gatataccat gggcagcagc catcatcatc atcatcacag cagcggcctg gtgccgcgcg
      121 gcagccatat ggctagctgg agccatccgc agtttgaaaa aggtggtggt agcggtggtg
      181 gttcaggtgg tagtgcatgg tcacaccctc agtttgagaa aatgtcggac tcagaagtca
      241 atcaagaagc taagccagag gtcaagccag aagtcaagcc tgagactcac atcaatttaa
      301 aggtgtccga tggatcttca gagatcttct tcaagatcaa aaagaccact cctttaagaa
      361 ggctgatgga agcgttcgct aaaagacagg gtaaggaaat ggactcctta agattcttgt
      421 acgacggtat tagaatccaa gctgatcaga cccctgaaga tttggacatg gaggataacg
      481 atattattga ggctcacaga gaacagattg gtggatctat gtctaaggag tgcaagaagc
      541 agcgccagga gaagaagcgg agactgcaga aggccaattt ctccatctct ctgaccggca
      601 agcacgtgtt cggcgcctac tttaatatgg ccagaacaaa ctttgtgaaa accatcaact
      661 acatcctgcc tatcgccggc gtgaggggca actatagcga gaatcagatc aacaagatgc
      721 tgcacgcact gttcctgatc caggcaggac gcaatgagga gctgaccaca gagcagaagc
      781 agtgggagaa gaagctgcgg ctgaacccag agcagcagac caagtttcag aagctgctgt
      841 tcaagcactt tccagtgctg ggaccaatga tggcagacgt ggcagatcac aaggcctatc
      901 tgaacaagaa gaagagcacc gtgcagacag aggacgagac attcgccatg ctgaagggcg
      961 tgtccctggc cgactgcctg gatatcatct gtctgatggc cgataccctg acagagtgta
     1021 gaaacttcta cacacacaag gacccttata acaagccaag ccagctggcc gatcagtacc
     1081 tgcaccagga gatgatcgcc aagaagctgg acaaggtggt ggtggcctcc aggcgcatcc
     1141 tgaaggatag ggagggcctg tctgtgaatg aggtggagtt cctgaccggc atcgatcacc
     1201 tgcaccagga ggtgctgaag gacgagttcg gcaacgccaa ggtgaaggat ggcaaagtga
     1261 tgaaaacctt cgtggagtac gacgacttct acttcaagat ctccggcaag cggctggtga
     1321 atggctacac agtgaccaca aaggacgata agcccgtgaa tgtgaacacc atgctgcctg
     1381 ccctgtctga cttcggcctg ctgtacttct gcgtgctgtt tctgagcaag ccctatgcca
     1441 agctgtttat cgatgaggtg cgcctgttcg agtactctcc ttttgacgat aaggagaaca
     1501 tgatcatgtc tgagatgctg agcatctatc ggatcagaac accccggctg cacaagatcg
     1561 actcccacga ttctaaggcc accctggcca tggacatctt cggcgagctg cggagatgtc
     1621 ctatggagct gtataacctg ctggacaaga acgccggcca gccattcttt cacgatgagg
     1681 tgaagcaccc aaactctcac acccccgacg tgagcaagcg gctgagatac gacgatcgct
     1741 ttcctacact ggccctgcgg tatatcgatg agacagagct gttcaagcgg atcagatttc
     1801 agctgcagct gggcagcttc cgctacaagt tttatgataa ggagaattgc atcgacggcc
     1861 gggtgagagt gaggcgcatc cagaaggaga tcaacggcta cggcagaatg caggaggtgg
     1921 ccgacaagag gatggataag tggggcgacc tgatccagaa gagggaggag cgcagcgtga
     1981 agctggagca cgaggagctg tatatcaatc tggaccagtt cctggaggac accgccgatt
     2041 ccacacctta cgtgaccgat agacggcccg cctataatat ccacgccaac cggatcggcc
     2101 tgtactggga ggactcccag aatcctaagc agtacaaggt gtttgatgag aacggcatgt
     2161 atatccccga gctggtggtg accgaggaca agaaggcccc tatcaagatg ccagcaccta
     2221 ggtgtgcact gagcgtgtac gacctgccag ccatgctgtt ctacgagtat ctgcgggagc
     2281 agcaggataa cgagtttccc agcgccgagc aggtcatcat cgagtacgag gacgattatc
     2341 gcaagttctt taaggccgtg gccgagggca agctgaagcc cttcaagcgg cccaaggagt
     2401 tcagggactt tctgaagaag gagtacccaa agctgcggat ggccgatatc cccaagaagc
     2461 tgcagctgtt tctgtgcagc cacggcctgt gctacaacaa taagcccgag acagtgtatg
     2521 agcggctgga cagactgacc ctgcagcacc tggaggagag agagctgcac atccagaaca
     2581 ggctggagca ctaccagaag gacagagata tgatcggcaa taaggacaac cagtatggca
     2641 agaagagctt ctccgatgtg cgccacggcg ccctggccag atacctggca cagagcatga
     2701 tggagtggca gcccacaaag ctgaaggata aggagaaggg ccacgacaag ctgaccggcc
     2761 tgaattataa cgtgctgaca gcctacctgg caacctatgg ccacccacag gtgcctgagg
     2821 agggcttcac ccctagaaca ctggagcagg tgctgatcaa tgcccacctg atcggcggct
     2881 ccaatccaca cccctttatc aacaaggtgc tggccctggg caataggaac atcgaggagc
     2941 tgtacctgca ctatctggag gaggagctga agcacatcag gtcccgcatc cagtctctga
     3001 gctccaaccc tagcgacaag gccctgtccg ccctgccatt catccaccac gatcggatga
     3061 gataccacga gcgcaccagc gaggagatga tggccctggc cgcacggtat accacaatcc
     3121 agctgccaga cggcctgttt acaccctaca tcctggagat cctgcagaag cactataccg
     3181 agaacagcga tctgcagaac gccctgtctc aggacgtgcc tgtgaagctg aatccaacct
     3241 gcaacgccgc ctacctgatc acactgttct atcagaccgt gctgaaggac aatgcccagc
     3301 ccttctacct gtccgataag acctatacaa gaaacaagga cggcgagaag gccgagtctt
     3361 tcagctttaa gagggcctac gagctgttct ctgtgctgaa caataacaag aaggacacat
     3421 tcccctttga gatgatccct ctgtttctga ccagcgatga gatccaggag agactgtccg
     3481 ccaagctgct ggacggcgat ggcaatcctg tgccagaagt gggagagaag ggcaagccag
     3541 ccacagacag ccagggcaac accatctgga agaggcgcat ctactccgag gtggacgatt
     3601 atgccgagaa gctgaccgac cgcgatatga agatctcctt caagggcgag tgggagaagc
     3661 tgcctcggtg gaagcaggat aagatcatca agcggcggga cgagacacgg cggcagatgc
     3721 gggacgagct gctgcagagg atgccacgct acatccggga catcaaggat aatgagagga
     3781 cactgcggag atataagacc caggatatgg tgctgttcct gctggccgag aagatgttta
     3841 ccaatatcat ctccgagcag tctagcgagt tcaactggaa gcagatgagg ctgtctaagg
     3901 tgtgcaacga ggccttcctg agacagaccc tgaccttccg ggtgcccgtg acagtgggcg
     3961 agacaacaat ctacgtggag caggagaata tgtctctgaa gaactacggc gagttctatc
     4021 gctttctgac agacgatcgg ctgatgagcc tgctgaataa catcgtggag acactgaagc
     4081 ccaatgagaa cggcgacctg gtcatcagac acacagacct gatgagcgag ctggccgcct
     4141 acgaccagta taggtctacc atcttcatgc tgatccagag catcgagaac ctgatcatca
     4201 caaataacgc cgtgctggac gatccagacg ccgatggctt ctgggtgaga gaggatctgc
     4261 ccaagaggaa taactttgcc tccctgctgg agctgatcaa tcagctgaat aacgtggagc
     4321 tgaccgacga tgagcgcaag ctgctggtgg ccatccggaa cgccttcagc cacaattcct
     4381 acaacatcga tttttccctg atcaaggacg tgaagcacct gcccgaggtg gccaagggca
     4441 tcctgcagca cctgcagtct atgctgggcg tggagatcac caagtaaggc cgcactcgag
     4501 gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt
     4561 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat
     4621 cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga
     4681 cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt
     4741 agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat
     4801 gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     4861 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     4921 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     4981 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     5041 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5101 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5161 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5221 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     5281 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5341 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5401 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     5461 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     5521 gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc
     5581 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     5641 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     5701 gctgataaat ctggagccgg tgagcgtggg tcacgcggta tcattgcagc actggggcca
     5761 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     5821 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     5881 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     5941 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6001 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6061 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6121 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6181 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6241 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6301 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6361 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6421 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6481 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6541 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6601 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     6661 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     6721 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     6781 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
     6841 acgcatctgt gcggtatttc acaccgcaat ggtgcactct cagtacaatc tgctctgatg
     6901 ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc
     6961 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     7021 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     7081 accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca
     7141 gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg
     7201 gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc
     7261 cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct
     7321 cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca
     7381 actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt
     7441 cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg
     7501 gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc
     7561 gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt
     7621 tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg
     7681 ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga
     7741 gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt
     7801 ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa
     7861 tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg
     7921 cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg
     7981 ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc aatgatcgaa
     8041 gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc
     8101 tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc
     8161 ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg
     8221 tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca
     8281 gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc
     8341 atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc
     8401 tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc
     8461 cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgagatccc
     8521 ggtgcctaat gagtgagcta acttacatta attgcgttgc gctcactgcc cgctttccag
     8581 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     8641 ttgcgtattg ggcgccaggg tggtttttct tttcaccagt gagacgggca acagctgatt
     8701 gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag
     8761 caggcgaaaa tcctgtttga tggtggttaa cggcgggata taacatgagc tgtcttcggt
     8821 atcgtcgtat cccactaccg agatatccgc accaacgcgc agcccggact cggtaatggc
     8881 gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc
     8941 ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg
     9001 ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag
     9061 acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc
     9121 gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat
     9181 gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac
     9241 agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc
     9301 gagaagattg tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac
     9361 caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg
     9421 cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag
     9481 ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc
     9541 ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga
     9601 gacaccggca tactctgcga catcgtataa cgttactggt ttcacattca ccaccctgaa
     9661 ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgcc attcgatggt
     9721 gtccgggatc tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta
     9781 ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca
     9841 acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc
     9901 cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg
     9961 cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga
    10021 tcccgcgaaa ttaatacgac tcactatagg
//