| 质粒名称: | pBV-Luc wt MBS1-4 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 16564 |
| 存储实验室: | Bert Vogelstein |
|
载体骨架基本信息 |
|
| 载体名称: | pBV-Luc |
| 载体出品公司: | Vogelstein Lab |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4867 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，荧光素酶 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | c-MYC binding sites |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | Luciferase（主干上的C末端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | KpnI(未破坏) |
| 3' 克隆位点: | EcoRI (未破坏) |
| 5' 测序引物 | RVprimer3 |
| 3' 测序引物 | LucNrev |
|
文献引用方法 |
|
| 材料和方法部分: | pBV-Luc wt MBS1-4 was a gift from Bert Vogelstein (Addgene plasmid # 16564 ; http://n2t.net/addgene:16564 ; RRID:Addgene_16564) |
| 参考文献部分: | Identification of CDK4 as a target of c-MYC. Hermeking H, Rago C, Schuhmacher M, Li Q, Barrett JF, Obaya AJ, O'Connell BC, Mateyak MK, Tam W, Kohlhuber F, Dang CV, Sedivy JM, Eick D, Vogelstein B, Kinzler KW. Proc Natl Acad Sci U S A. 2000 Feb 29. 97(5):2229-34. 10.1073/pnas.050586197 PubMed 10688915 |
买家导航
pBV-Luc wt MBS1-4载体质粒基本信息
pBV-Luc wt MBS1-4质粒图谱载体图谱和pBV-Luc wt MBS1-4载体序列质粒序列多克隆位点信息
pBV-Luc wt MBS1-4质粒载体简介
pBV-Luc wt MBS1-4质粒序列载体序列
LOCUS pBV-Luc wt MBS1-4 5025 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pBV-Luc wt MBS1-4
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5025)
AUTHORS Hermeking H, Rago C, Schuhmacher M, Li Q, Barrett JF, Obaya AJ,
O'Connell BC, Mateyak MK, Tam W, Kohlhuber F, Dang CV, Sedivy JM,
Eick D, Vogelstein B, Kinzler KW
TITLE Identification of CDK4 as a target of c-MYC.
JOURNAL Proc Natl Acad Sci U S A. 2000 Feb 29. 97(5):2229-34.
PUBMED 10688915
REFERENCE 2 (bases 1 to 5025)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5025
/organism="synthetic DNA construct"
/mol_type="other DNA"
regulatory 289..298
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 295..1947
/codon_start=1
/gene="luc+"
/product="firefly luciferase"
/label=luciferase
/note="enhanced luc+ version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
primer_bind complement(361..379)
/label=LucNrev
/note="Firefly luciferase, reverse primer"
polyA_signal 1988..2109
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1999..2018)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 2053..2072
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(2357..2374)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2528..3116)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2608..2627)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3287..4147)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 3910..3929
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4148..4252)
/gene="bla"
/label=AmpR promoter
rep_origin 4279..4734
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4366..4385)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4576..4597
/label=F1ori-F
/note="F1 origin, forward primer"
polyA_signal 4865..4913
/note="synthetic polyadenylation signal"
misc_feature 4927..5018
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
primer_bind 4967..4986
/label=RVprimer3
/note="pGL3 vector, forward primer"
ORIGIN
1 ggtaccgggt tgttggcagc cagtcacgtg cccgccgcgt agccacacct ctgctcctca
61 gagcaatgtc aagcggtcac gtgtgatagc aacagatcac gtggctgcca tcgcccctcc
121 gcccccttac actcttcgcc ctcctcccag tcgaagcacc tcctgtccgc ccctcagcgc
181 catgggtggc ggtcacgtgc ccagaacgtc cggaattcgg gtatataatg gatccggtat
241 cgagatctgc gatctaagta agcttggcat tccggtactg ttggtaaagc caccatggaa
301 gacgccaaaa acataaagaa aggcccggcg ccattctatc cgctggaaga tggaaccgct
361 ggagagcaac tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt
421 acagatgcac atatcgaggt ggacatcact tacgctgagt acttcgaaat gtccgttcgg
481 ttggcagaag ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt
541 gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt
601 gcgcccgcga acgacattta taatgaacgt gaattgctca acagtatggg catttcgcag
661 cctaccgtgg tgttcgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaag
721 ctcccaatca tccaaaaaat tattatcatg gattctaaaa cggattacca gggatttcag
781 tcgatgtaca cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgtg
841 ccagagtcct tcgataggga caagacaatt gcactgatca tgaactcctc tggatctact
901 ggtctgccta aaggtgtcgc tctgcctcat agaactgcct gcgtgagatt ctcgcatgcc
961 agagatccta tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca
1021 ttccatcacg gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc
1081 gtcttaatgt atagatttga agaagagctg tttctgagga gccttcagga ttacaagatt
1141 caaagtgcgc tgctggtgcc aaccctattc tccttcttcg ccaaaagcac tctgattgac
1201 aaatacgatt tatctaattt acacgaaatt gcttctggtg gcgctcccct ctctaaggaa
1261 gtcggggaag cggttgccaa gaggttccat ctgccaggta tcaggcaagg atatgggctc
1321 actgagacta catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc
1381 ggtaaagttg ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg
1441 ggcgttaatc aaagaggcga actgtgtgtg agaggtccta tgattatgtc cggttatgta
1501 aacaatccgg aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac
1561 atagcttact gggacgaaga cgaacacttc ttcatcgttg accgcctgaa gtctctgatt
1621 aagtacaaag gctatcaggt ggctcccgct gaattggaat ccatcttgct ccaacacccc
1681 aacatcttcg acgcaggtgt cgcaggtctt cccgacgatg acgccggtga acttcccgcc
1741 gccgttgttg ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc
1801 gccagtcaag taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta
1861 ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc
1921 aagaagggcg gaaagatcgc cgtgtaattc tagagtcggg gcggccggcc gcttcgagca
1981 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
2041 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
2101 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
2161 gaggtttttt aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatccgtcg
2221 accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
2281 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
2341 agcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
2401 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
2461 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
2521 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
2581 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
2641 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
2701 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
2761 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
2821 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
2881 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
2941 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
3001 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
3061 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
3121 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
3181 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
3241 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
3301 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
3361 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
3421 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
3481 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
3541 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
3601 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
3661 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
3721 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
3781 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
3841 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
3901 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
3961 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
4021 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
4081 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
4141 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
4201 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
4261 cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
4321 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
4381 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
4441 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
4501 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
4561 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
4621 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
4681 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttgccatt
4741 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
4801 gccagcccaa gctaccatga taagtaagta atattaaggt acgggaggta cttggagcgg
4861 ccgcaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat
4921 agtactaaca tacgctctcc atcaaaacaa aacgaaacaa aacaaactag caaaataggc
4981 tgtccccagt gcaagtgcag gtgccagaac atttctctat cgata
//
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