pBABEpuro HA Brca1

LOCUS       pBABEpuro HA Brca1               10749 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pBABEpuro HA Brca1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10749)
  AUTHORS   Cortez D, Wang Y, Qin J, Elledge SJ
  TITLE     Requirement of ATM-dependent phosphorylation of brca1 in the DNA 
            damage response to double-strand breaks.
  JOURNAL   Science. 1999 Nov 5. 286(5442):1162-6.
  PUBMED    10550055
REFERENCE   2  (bases 1 to 10749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10749
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(11..28)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(262..281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(930..1790)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1553..1572
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1791..1895)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             1926..2395
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    2460..2659
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             2860..3276
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     3206..3228
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3244..3260
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             3347..3373
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(8997..9017)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9002..9331
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9182..9317
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9244..9263
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             9341..9940
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9341..9360)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9837..9857
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             10161..10630
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt gcggccgcaa atcaatctaa
      901 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
      961 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     1021 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     1081 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     1141 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     1201 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     1261 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     1321 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     1381 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     1441 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     1501 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     1561 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     1621 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     1681 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     1741 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     1801 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     1861 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     1921 gcagcctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg
     1981 gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc
     2041 ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt
     2101 tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt
     2161 atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc
     2221 tcaataaaag agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc
     2281 ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg
     2341 ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatttgg
     2401 gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca ccgggaggca
     2461 agctggccag caacttatct gtgtctgtcc gattgtctag tgtctatgac tgattttatg
     2521 cgcctgcgtc ggtactagtt agctaactag ctctgtatct ggcggacccg tggtggaact
     2581 gacgagttct gaacacccgg ccgcaaccct gggagacgtc ccagggactt tgggggccgt
     2641 ttttgtggcc cgacctgagg aagggagtcg atgtggaatc cgaccccgtc aggatatgtg
     2701 gttctggtag gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg
     2761 gtttggaacc gaagccgcgc gtcttgtctg ctgcagcgct gcagcatcgt tctgtgttgt
     2821 ctctgtctga ctgtgtttct gtatttgtct gaaaattagg gccagactgt taccactccc
     2881 ttaagtttga ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta
     2941 gatgtcaaga agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc
     3001 ggatggccgc gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc
     3061 ttttcacctg gcccgcatgg acacccagac caggtcccct acatcgtgac ctgggaagcc
     3121 ttggcttttg acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct
     3181 cttcctccat ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc
     3241 tccctttatc cagccctcac tccttctcta ggcgccggcc ggatcccagt gtggtggtac
     3301 gtaggaattc gccagcacag tggtcgaccc caagcttacc atggcctacc cctacgacgt
     3361 gcccgactac gcctccctcg gatccatgga tttatctgct cttcgcgttg aagaagtaca
     3421 aaatgtcatt aatgctatgc agaaaatctt agagtgtccc atctgtctgg agttgatcaa
     3481 ggaacctgtc tccacaaagt gtgaccacat attttgcaaa ttttgcatgc tgaaacttct
     3541 caaccagaag aaagggcctt cacagtgtcc tttatgtaag aatgatataa ccaaaaggag
     3601 cctacaagaa agtacgagat ttagtcaact tgttgaagag ctattgaaaa tcatttgtgc
     3661 ttttcagctt gacacaggtt tggagtatgc aaacagctat aattttgcaa aaaaggaaaa
     3721 taactctcct gaacatctaa aagatgaagt ttctatcatc caaagtatgg gctacagaaa
     3781 ccgtgccaaa agacttctac agagtgaacc cgaaaatcct tccttgcagg aaaccagtct
     3841 cagtgtccaa ctctctaacc ttggaactgt gagaactctg aggacaaagc agcggataca
     3901 acctcaaaag acgtctgtct acattgaatt gggatctgat tcttctgaag ataccgttaa
     3961 taaggcaact tattgcagtg tgggagatca agaattgtta caaatcaccc ctcaaggaac
     4021 cagggatgaa atcagtttgg attctgcaaa aaaggctgct tgtgaatttt ctgagacgga
     4081 tgtaacaaat actgaacatc atcaacccag taataatgat ttgaacacca ctgagaagcg
     4141 tgcagctgag aggcatccag aaaagtatca gggtagttct gtttcaaact tgcatgtgga
     4201 gccatgtggc acaaatactc atgccagctc attacagcat gagaacagca gtttattact
     4261 cactaaagac agaatgaatg tagaaaaggc tgaattctgt aataaaagca aacagcctgg
     4321 cttagcaagg agccaacata acagatgggc tggaagtaag gaaacatgta atgataggcg
     4381 gactcccagc acagaaaaaa aggtagatct gaatgctgat cccctgtgtg agagaaaaga
     4441 atggaataag cagaaactgc catgctcaga gaatcctaga gatactgaag atgttccttg
     4501 gataacacta aatagcagca ttcagaaagt taatgagtgg ttttccagaa gtgatgaact
     4561 gttaggttct gatgactcac atgatgggga gtctgaatca aatgccaaag tagctgatgt
     4621 attggacgtt ctaaatgagg tagatgaata ttctggttct tcagagaaaa tagacttact
     4681 ggccagtgat cctcatgagg ctttaatatg taaaagtgaa agagttcact ccaaatcagt
     4741 agagagtaat attgaagaca aaatatttgg gaaaacctat cggaagaagg caagcctccc
     4801 caacttaagc catgtaactg aaaatctaat tataggagca tttgttactg agccacagat
     4861 aatacaagag cgtcccctca caaataaatt aaagcgtaaa aggagaccta catcaggcct
     4921 tcatcctgag gattttatca agaaagcaga tttggcagtt caaaagactc ctgaaatgat
     4981 aaatcaggga actaaccaaa cggagcagaa tggtcaagtg atgaatatta ctaatagtgg
     5041 tcatgagaat aaaacaaaag gtgattctat tcagaatgag aaaaatccta acccaataga
     5101 atcactcgaa aaagaatctg ctttcaaaac gaaagctgaa cctataagca gcagtataag
     5161 caatatggaa ctcgaattaa atatccacaa ttcaaaagca cctaaaaaga ataggctgag
     5221 gaggaagtct tctaccaggc atattcatgc gcttgaacta gtagtcagta gaaatctaag
     5281 cccacctaat tgtactgaat tgcaaattga tagttgttct agcagtgaag agataaagaa
     5341 aaaaaagtac aaccaaatgc cagtcaggca cagcagaaac ctacaactca tggaaggtaa
     5401 agaacctgca actggagcca agaagagtaa caagccaaat gaacagacaa gtaaaagaca
     5461 tgacagtgat actttcccag agctgaagtt aacaaatgca cctggttctt ttactaagtg
     5521 ttcaaatacc agtgaactta aagaatttgt caatcctagc cttccaagag aagaaaaaga
     5581 agagaaacta gaaacagtta aagtgtctaa taatgctgaa gaccccaaag atctcatgtt
     5641 aagtggagaa agggttttgc aaactgaaag atctgtagag agtagcagta tttcactggt
     5701 acctggtact gattatggca ctcaggaaag tatctcgtta ctggaagtta gcactctagg
     5761 gaaggcaaaa acagaaccaa ataaatgtgt gagtcagtgt gcagcatttg aaaaccccaa
     5821 gggactaatt catggttgtt ccaaagataa tagaaatgac acagaaggct ttaagtatcc
     5881 attgggacat gaagttaacc acagtcggga aacaagcata gaaatggaag aaagtgaact
     5941 tgatgctcag tatttgcaga atacattcaa ggtttcaaag cgccagtcat ttgctctgtt
     6001 ttcaaatcca ggaaatgcag aagaggaatg tgcaacattc tctgcccact ctgggtcctt
     6061 aaagaaacaa agtccaaaag tcacttttga atgtgaacaa aaggaagaaa atcaaggaaa
     6121 gaatgagtct aatatcaagc ctgtacagac agttaatatc actgcaggct ttcctgtggt
     6181 tggtcagaaa gataagccag ttgataatgc caaatgtagt atcaaaggag gctctaggtt
     6241 ttgtctatca tctcagttca gaggcaacga aactggactc attactccaa ataaacatgg
     6301 acttttacaa aacccatatc gtataccacc actttttccc atcaagtcat ttgttaaaac
     6361 taaatgtaag aaaaatctgc tagaggaaaa ctttgaggaa cattcaatgt cacctgaaag
     6421 agaaatggga aatgagaaca ttccaagtac agtgagcaca attagccgta ataacattag
     6481 agaaaatgtt tttaaaggag ccagctcaag caatattaat gaagtaggtt ccagtactaa
     6541 tgaagtgggc tccagtatta atgaaatagg ttccagtgat gaaaacattc aagcagaact
     6601 aggtagaaac agagggccaa aattgaatgc tatgcttaga ttaggggttt tgcaacctga
     6661 ggtctataaa caaagtcttc ctggaagtaa ttgtaagcat cctgaaataa aaaagcaaga
     6721 atatgaagaa gtagttcaga ctgttaatac agatttctct ccatatctga tttcagataa
     6781 cttagaacag cctatgggaa gtagtcatgc atctcaggtt tgttctgaga cacctgatga
     6841 cctgttagat gatggtgaaa taaaggaaga tactagtttt gctgaaaatg acattaagga
     6901 aagttctgct gtttttagca aaagcgtcca gagaggagag cttagcagga gtcctagccc
     6961 tttcacccat acacatttgg ctcagggtta ccgaagaggg gccaagaaat tagagtcctc
     7021 agaagagaac ttatctagtg aggatgaaga gcttccctgc ttccaacact tgttatttgg
     7081 taaagtaaac aatatacctt ctcagtctac taggcatagc accgttgcta ccgagtgtct
     7141 gtctaagaac acagaggaga atttattatc attgaagaat agcttaaatg actgcagtaa
     7201 ccaggtaata ttggcaaagg catctcagga acatcacctt agtgaggaaa caaaatgttc
     7261 tgctagcttg ttttcttcac agtgcagtga attggaagac ttgactgcaa atacaaacac
     7321 ccaggatcct ttcttgattg gttcttccaa acaaatgagg catcagtctg aaagccaggg
     7381 agttggtctg agtgacaagg aattggtttc agatgatgaa gaaagaggaa cgggcttgga
     7441 agaaaataat caagaagagc aaagcatgga ttcaaactta ggtgaagcag catctgggtg
     7501 tgagagtgaa acaagcgtct ctgaagactg ctcagggcta tcctctcaga gtgacatttt
     7561 aaccactcag cagagggata ccatgcaaca taacctgata aagctccagc aggaaatggc
     7621 tgaactagaa gctgtgttag aacagcatgg gagccagcct tctaacagct acccttccat
     7681 cataagtgac tcctctgccc ttgaggacct gcgaaatcca gaacaaagca catcagaaaa
     7741 agcagtatta acttcacaga aaagtagtga ataccctata agccagaatc cagaaggcct
     7801 ttctgctgac aagtttgagg tgtctgcaga tagttctacc agtaaaaata aagaaccagg
     7861 agtggaaagg tcatcccctt ctaaatgccc atcattagat gataggtggt acatgcacag
     7921 ttgctctggg agtcttcaga atagaaacta cccatctcaa gaggagctca ttaaggttgt
     7981 tgatgtggag gagcaacagc tggaagagtc tgggccacac gatttgacgg aaacatctta
     8041 cttgccaagg caagatctag agggaacccc ttacctggaa tctggaatca gcctcttctc
     8101 tgatgaccct gaatctgatc cttctgaaga cagagcccca gagtcagctc gtgttggcaa
     8161 cataccatct tcaacctctg cattgaaagt tccccaattg aaagttgcag aatctgccca
     8221 gggtccagct gctgctcata ctactgatac tgctgggtat aatgcaatgg aagaaagtgt
     8281 gagcagggag aagccagaat tgacagcttc aacagaaagg gtcaacaaaa gaatgtccat
     8341 ggtggtgtct ggcctgaccc cagaagaatt tatgctcgtg tacaagtttg ccagaaaaca
     8401 ccacatcact ttaactaatc taattactga agagactact catgttgtta tgaaaacaga
     8461 tgctgagttt gtgtgtgaac ggacactgaa atattttcta ggaattgcgg gaggaaaatg
     8521 ggtagttagc tatttctggg tgacccagtc tattaaagaa agaaaaatgc tgaatgagca
     8581 tgattttgaa gtcagaggag atgtggtcaa tggaagaaac caccaaggtc caaagcgagc
     8641 aagagaatcc caggacagaa agatcttcag ggggctagaa atctgttgct atgggccctt
     8701 caccaacatg cccacagatc aactggaatg gatggtacag ctgtgtggtg cttctgtggt
     8761 gaaggagctt tcatcattca cccttggcac aggtgtccac ccaattgtgg ttgtgcagcc
     8821 agatgcctgg acagaggaca atggcttcca tgcaattggg cagatgtgtg aggcacctgt
     8881 ggtgacccga gagtgggtgt tggacagtgt agcactctac cagtgccagg agctggacac
     8941 ctacctgata ccccagatcc cccacagcca ctactgagat atcggctcga ccctgtggaa
     9001 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     9061 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     9121 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     9181 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     9241 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     9301 aggctttttt ggaggcctag gcttttgcaa aaagcttacc atgaccgagt acaagcccac
     9361 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     9421 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     9481 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     9541 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     9601 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     9661 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     9721 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     9781 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     9841 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     9901 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca
     9961 gcgcccgacc gaaaggagcg cacgacccca tgcatcgata aaataaaaga ttttatttag
    10021 tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt
    10081 aacgccattt tgcaaggcat ggaaaaatac ataactgaga atagagaagt tcagatcaag
    10141 gtcaggaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc
    10201 ctgccccggc tcagggccaa gaacagatgg aacagctgaa tatgggccaa acaggatatc
    10261 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc
    10321 cagccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa
    10381 atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc
    10441 ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc
    10501 gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga
    10561 cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg
    10621 gggtctttca catgcagcat gtatcaaaat taatttggtt ttttttctta agtatttaca
    10681 ttaaatggcc atagttgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
    10741 ttgggcgct
//