| 质粒名称: | T7opt in pCAGGS |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 65974 |
| 存储实验室: | Benhur Lee |
|
载体骨架基本信息 |
|
| 载体名称: | pCAGGS |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4729 bp |
| 完整质粒大小: | 7381 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | T7-opt,T7 RNA polymerase |
| 物种来源: | bacteriophage |
| 插入大小: | 2652 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | T7opt in pCAGGS was a gift from Benhur Lee (Addgene plasmid # 65974 ; http://n2t.net/addgene:65974 ; RRID:Addgene_65974) |
| 参考文献部分: | Efficient reverse genetics reveals genetic determinants of budding and fusogenic differences between Nipah and Hendra viruses and enables real-time monitoring of viral spread in small animal models of henipavirus infection. Yun T, Park A, Hill TE, Pernet O, Beaty SM, Juelich TL, Smith JK, Zhang L, Wang YE, Vigant F, Gao J, Wu P, Lee B, Freiberg AN. J Virol. 2015 Jan 15;89(2):1242-53. doi: 10.1128/JVI.02583-14. Epub 2014 Nov 12. 10.1128/JVI.02583-14 PubMed 25392218 |
买家导航
T7opt in pCAGGS载体质粒基本信息
T7opt in pCAGGS质粒图谱载体图谱和T7opt in pCAGGS载体序列质粒序列多克隆位点信息
T7opt in pCAGGS质粒载体简介
T7opt in pCAGGS质粒序列载体序列
LOCUS T7opt in pCAGGS 7381 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Human codon-optimized T7 polymerase.
ACCESSION .
VERSION .
KEYWORDS T7opt in pCAGGS
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7381)
AUTHORS Yun T, Park A, Hill TE, Pernet O, Beaty SM, Juelich TL, Smith JK,
Zhang L, Wang YE, Vigant F, Gao J, Wu P, Lee B, Freiberg AN
TITLE Efficient reverse genetics reveals genetic determinants of budding
and fusogenic differences between Nipah and Hendra viruses and
enables real-time monitoring of viral spread in small animal models
of henipavirus infection.
JOURNAL J Virol. 2015 Jan 15;89(2):1242-53. doi: 10.1128/JVI.02583-14. Epub
2014 Nov 12.
PUBMED 25392218
REFERENCE 2 (bases 1 to 7381)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7381
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 368..645
/label=chicken beta-actin promoter
intron 647..1664
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 1588..1610
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 1672..1691
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
primer_bind complement(4385..4404)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_site 4450..4505
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal"
primer_bind complement(4504..4523)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(4866..4882)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4866..4882)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4879..4901)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4890..4906
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4914..4944)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4959..4980
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 5039..5234
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 5085..5220
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5147..5166
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
polyA_signal 5240..5374
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5277..5296)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5331..5350
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(5442..5459)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5613..6201)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5693..5712)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6372..7232)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6995..7014
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7233..7337)
/gene="bla"
/label=AmpR promoter
enhancer 7368..366
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
ORIGIN
1 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
361 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
421 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
481 gggggggggc gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg
541 agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg
601 cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcggggag tcgctgcgac
661 gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac
721 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt
781 agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc
841 tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg
901 tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg
961 cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc
1021 ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt
1081 gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag
1141 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
1201 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg
1261 ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
1321 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
1381 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
1441 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
1501 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
1561 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
1621 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
1681 tggttattgt gctgtctcat cattttggca aagaattcgc caccatgaac accatcaata
1741 ttgccaagaa cgacttttct gatatcgagc tggccgctat tccattcaat acactggctg
1801 accactacgg agagcggctg gcccgcgaac agctggctct ggagcatgaa agctatgaga
1861 tgggagaagc ccgattcagg aagatgtttg agaggcagct gaaagctggg gaagtggcag
1921 acaacgcagc cgctaagcca ctgattacca cactgctgcc caaaatgatc gccagaatta
1981 atgattggtt cgaggaagtg aaggcaaaac gaggaaagag gcctaccgcc ttccagtttc
2041 tgcaggagat caagccagaa gctgtggcat acatcaccat caagactacc ctggcatgcc
2101 tgacaagcgc cgacaacaca actgtgcagg cagtcgcttc cgcaatcgga cgagctattg
2161 aggacgaagc acgctttggg agaatccggg atctggaggc caagcacttc aagaaaaacg
2221 tggaggaaca gctgaacaag agggtggggc atgtctataa gaaggccttc atgcaggtgg
2281 tcgaggccga catgctgtca aagggactgc tgggaggaga ggcctggagc tcctggcaca
2341 aagaagatag catccatgtg ggagtccgct gcatcgagat gctgattgaa tctactggga
2401 tggtgagtct gcaccgacag aacgccggcg tggtcggaca ggactctgag acaatcgaac
2461 tggctcccga gtatgccgaa gctattgcaa ctagagccgg agctctggca gggatcagtc
2521 caatgttcca gccctgcgtg gtccccccta agccttggac tggcatcacc gggggcggat
2581 actgggctaa tggaaggaga ccactggcac tggtgcgaac acactctaag aaagccctga
2641 tgagatacga ggatgtctat atgcccgaag tgtataaggc catcaacatt gctcagaata
2701 cagcatggaa aattaacaag aaagtgctgg ccgtcgctaa tgtgatcact aagtggaaac
2761 attgtcccgt ggaggacatc cctgccattg aacgggagga actgcctatg aagccagagg
2821 acatcgatat gaaccctgaa gctctgaccg catggaagcg cgcagccgct gcagtgtaca
2881 gaaaggataa agcccggaag tcccggcgca tttctctgga gttcatgctg gaacaggcca
2941 acaagtttgc taatcacaaa gcaatctggt tcccctacaa catggactgg cgcggacgag
3001 tctatgccgt gagcatgttc aaccctcagg ggaatgatat gacaaagggc ctgctgactc
3061 tggctaaggg gaaaccaatt gggaaggagg gctactattg gctgaaaatc cacggggcca
3121 attgcgctgg cgtcgacaag gtgccattcc ccgagaggat caagttcatc gaggaaaacc
3181 atgaaaatat tatggcatgt gccaagtctc ccctggagaa cacatggtgg gccgaacagg
3241 atagtccttt ctgctttctg gccttctgtt ttgagtacgc tggagtgcag caccatgggc
3301 tgagttataa ttgctccctg ccactggcct ttgacggctc ttgtagtgga atccagcact
3361 tctccgcaat gctgagggat gaggtcggag gaagagcagt gaacctgctg ccatctgaga
3421 cagtgcagga catctacggc attgtcgcca agaaagtgaa tgagatcctg caggctgacg
3481 caattaacgg gactgataat gaggtggtca ccgtcacaga tgaaaacact ggcgagatca
3541 gcgaaaaggt gaaactggga accaaggccc tggctggaca gtggctggca tacggagtca
3601 cccgctcagt gacaaagcga agcgtgatga ccctggctta tggcagcaaa gagttcggct
3661 tcaggcagca ggtgctggaa gacaccatcc agccagccat tgattccgga aaggggctga
3721 tgtttacaca gcccaaccag gccgctggct acatggccaa gctgatctgg gagtcagtga
3781 gcgtcacagt ggtcgcagcc gtggaagcta tgaattggct gaagtccgct gcaaaactgc
3841 tggccgctga ggtgaaggac aagaaaactg gcgaaattct gaggaaaaga tgcgccgtcc
3901 actgggtgac ccctgatgga ttcccagtgt ggcaggagta taagaaaccc atccagacca
3961 gactgaacct gatgttcctg ggccagtttc ggctgcagcc tacaatcaac actaataagg
4021 acagtgagat tgatgctcat aaacaggaat cagggattgc acctaatttt gtgcacagcc
4081 aggacggctc ccatctgcgg aagactgtgg tctgggctca cgagaaatac ggcatcgaat
4141 ccttcgcact gattcatgac tcttttggaa ccatcccagc cgatgcagcc aacctgttca
4201 aggctgtccg cgagactatg gtggacacct acgaaagttg tgatgtgctg gccgacttct
4261 atgatcagtt tgctgaccag ctgcacgagt cacagctgga taagatgccc gcactgcctg
4321 ccaaaggcaa cctgaatctg agagacatcc tggagtccga tttcgcattt gcctgactcg
4381 aggatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga
4441 cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc
4501 tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg
4561 tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt tggctataaa
4621 gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat agaaaagcct
4681 tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct ttaacatccc
4741 taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga ctactcccag
4801 tcatagctgt ccctcttctc ttatggagat ccctcgacct gcagcccaag cttggcgtaa
4861 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
4921 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
4981 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gcggatccgc
5041 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
5101 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
5161 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
5221 taggcttttg caaaaagcta acttgtttat tgcagcttat aatggttaca aataaagcaa
5281 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
5341 caaactcatc aatgtatctt atcatgtctg gatccgctgc attaatgaat cggccaacgc
5401 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
5461 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
5521 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
5581 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
5641 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
5701 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
5761 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
5821 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
5881 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
5941 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
6001 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
6061 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
6121 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
6181 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
6241 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
6301 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
6361 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
6421 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
6481 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
6541 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
6601 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
6661 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
6721 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
6781 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
6841 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
6901 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
6961 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
7021 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
7081 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
7141 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
7201 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
7261 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
7321 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgggtcgac attgattatt
7381 g
//
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