| 质粒名称: | HBV 1.3-mer WT replicon |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 90517 |
| 存储实验室: | Wang-Shick Ryu |
|
载体骨架基本信息 |
|
| 载体名称: | pGEM-4Z |
| 载体出品公司: | promega |
| 空载体大小: | 6821bp |
| 完整质粒大小: | 6821bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | 1.3 units of the HBV genome |
| 物种来源: | Hepatitis B virus |
| GenBank编号 | DV01460 |
|
文献引用方法 |
|
| 材料和方法部分: | HBV 1.3-mer WT replicon was a gift from Wang-Shick Ryu (Addgene plasmid # 65459) |
| 参考文献部分: | DDX3 DEAD-Box RNA helicase inhibits hepatitis B virus reverse transcription by incorporation into nucleocapsids. Wang H, Kim S, Ryu WS. J Virol. 2009 Jun;83(11):5815-24. doi: 10.1128/JVI.00011-09. Epub 2009 Mar 18. 10.1128/JVI.00011-09 PubMed 19297497 |
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HBV 1.3-mer WT replicon载体质粒基本信息
HBV 1.3-mer WT replicon质粒图谱载体图谱和HBV 1.3-mer WT replicon载体序列质粒序列多克隆位点信息
HBV 1.3-mer WT replicon质粒载体简介
HBV 1.3-mer WT replicon质粒序列载体序列
LOCUS HBV 1.3-mer WT replicon 6821 bp DNA circular SYN 14-APR-2025
DEFINITION containing 1.3 units of the HBV genome (subtype ayw).
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6821)
AUTHORS Wang H, Kim S, Ryu WS
TITLE DDX3 DEAD-Box RNA helicase inhibits hepatitis B virus reverse
transcription by incorporation into nucleocapsids.
JOURNAL J Virol. 2009 Jun;83(11):5815-24. doi: 10.1128/JVI.00011-09. Epub
2009 Mar 18.
PUBMED 19297497
REFERENCE 2 (bases 1 to 6821)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1128/JVI.00011-09"; journalName: "J Virol"; date: "2009-06";
volume: "83"; issue: "11"; pages: "5815-24"
COMMENT Sequence Label: HBV 1.3-mer WT replicon
FEATURES Location/Qualifiers
source 1..6821
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 843..1688
/codon_start=1
/product="middle (pre-S2/S) envelope protein of hepatitis B
virus"
/label=HBsAg (pre-S2/S)
/note="hepatitis B surface antigen"
/translation="MQWNSTTFHQTLQDPRVRGLYFPAGGSSSGTVNPVLTTASPLSSI
FSRIGDPALNMENITSGFLGPLLVLQAGFFLLTRILTIPQSLDSWWTSLNFLGGTTVCL
GQNSQSPTSNHSPTSCPPTCPGYRWMCLRRFIIFLFILLLCLIFLLVLLDYQGMLPVCP
LIPGSSTTSTGPCRTCMTTAQGTSMYPSCCCTKPSDGNCTCIPIPSSWAFGKFLWEWAS
ARFSWLSLLVPFVQWFVGLSPTVWLSVIWMMWYWGPSLYSILSPFLPLLPIFFCLWVYI
"
primer_bind complement(2865..2884)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(2866..2884)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(2903..2919)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2903..2919)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(2916..2938)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 2927..2943
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(2951..2957,2958..2975,2976..2981))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2996..3017
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(3134..3151)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3305..3893)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3385..3404)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(4064..4855,4856..4924))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4687..4706
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4925..5029)
/gene="bla"
/label=AmpR promoter
primer_bind 5097..5115
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(5153..5175)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 5275..5294
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 5488..5510
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 5502..5519
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 5503..5519
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 5527..5545
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 5527..5544
/label=SP6
/note="SP6 promoter, forward primer"
ORIGIN
1 ccggagacta ctgttgttag acgacgaggc aggtccccta gaagaagaac tccctcgcct
61 cgcagacgaa ggtctcaatc gccgcgtcgc agaagatctc aatctcggga atctcaatgt
121 tagtattcct tggactcata aggtggggaa ctttactggg ctttattctt ctactgtacc
181 tgtctttaat cctcattgga aaacaccatc ttttcctaat atacatttac accaagacat
241 tatcaaaaaa tgtgaacagt ttgtaggccc actcacagtt aatgagaaaa gaagattgca
301 attgattatg cctgccaggt tttatccaaa ggttaccaaa tatttaccat tggataaggg
361 tattaaacct tattatccag aacatctagt taatcattac ttccaaacta gacactattt
421 acacactcta tggaaggcgg gtatattata taagagagaa acaacacata gcgcctcatt
481 ttgtgggtca ccatattctt gggaacaaga tctacagcat ggggcagaat ctttccacca
541 gcaatcctct gggattcttt cccgaccacc agttggatcc agccttcaga gcaaacaccg
601 caaatccaga ttgggacttc aatcccaaca aggacacctg gccagacgcc aacaaggtag
661 gagctggagc attcgggctg ggtttcaccc caccgcacgg aggccttttg gggtggagcc
721 ctcaggctca gggcatacta caaactttgc cagcaaatcc gcctcctgcc tccaccaatc
781 gccagtcagg aaggcagcct accccgctgt ctccaccttt gagaaacact catcctcagg
841 ccatgcagtg gaattccaca accttccacc aaactctgca agatcccaga gtgagaggcc
901 tgtatttccc tgctggtggc tccagttcag gaacagtaaa ccctgttctg actactgcct
961 ctcccttatc gtcaatcttc tcgaggattg gggaccctgc gctgaacatg gagaacatca
1021 catcaggatt cctaggaccc cttctcgtgt tacaggcggg gtttttcttg ttgacaagaa
1081 tcctcacaat accgcagagt ctagactcgt ggtggacttc tctcaatttt ctagggggaa
1141 ctaccgtgtg tcttggccaa aattcgcagt ccccaacctc caatcactca ccaacctctt
1201 gtcctccaac ttgtcctggt tatcgctgga tgtgtctgcg gcgttttatc atcttcctct
1261 tcatcctgct gctatgcctc atcttcttgt tggttcttct ggactatcaa ggtatgttgc
1321 ccgtttgtcc tctaattcca ggatcctcaa caaccagcac gggaccatgc cggacctgca
1381 tgactactgc tcaaggaacc tctatgtatc cctcctgttg ctgtaccaaa ccttcggacg
1441 gaaattgcac ctgtattccc atcccatcat cctgggcttt cggaaaattc ctatgggagt
1501 gggcctcagc ccgtttctcc tggctcagtt tactagtgcc atttgttcag tggttcgtag
1561 ggctttcccc cactgtttgg ctttcagtta tatggatgat gtggtattgg gggccaagtc
1621 tgtacagcat cttgagtccc tttttaccgc tgttaccaat tttcttttgt ctttgggtat
1681 acatttaaac cctaacaaaa caaagagatg gggttactct ctaaatttta tgggttatgt
1741 cattggatgt tatgggtcct tgccacaaga acacatcata caaaaaatca aagaatgttt
1801 tagaaaactt cctattaaca ggcctattga ttggaaagta tgtcaacgaa ttgtgggtct
1861 tttgggtttt gctgcccctt ttacacaatg tggttatcct gcgttgatgc ctttgtatgc
1921 atgtattcaa tctaagcagg ctttcacttt ctcgccaact tacaaggcct ttctgtgtaa
1981 acaatacctg aacctttacc ccgttgcccg gcaacggcca ggtctgtgcc aagtgtttgc
2041 tgacgcaacc cccactggct ggggcttggt catgggccat cagcgcatgc gtggaacctt
2101 ttcggctcct ctgccgatcc atactgcgga actcctagcc gcttgttttg ctcgcagcag
2161 gtctggagca aacattatcg ggactgataa ctctgttgtc ctatcccgca aatatacatc
2221 gtttccatgg ctgctaggct gtgctgccaa ctggatcctg cgcgggacgt cctttgttta
2281 cgtcccgtcg gcgctgaatc ctgcggacga cccttctcgg ggtcgcttgg gactctctcg
2341 tccccttctc cgtctgccgt tccgaccgac cacggggcgc acctctcttt acgcggactc
2401 cccgtctgtg ccttctcatc tgccggaccg tgtgcacttc gcttcacctc tgcacgtcgc
2461 atggagacca ccgtgaacgc ccaccaaata ttgcccaagg tcttacataa gaggactctt
2521 ggactctcag caatgtcaac gaccgacctt gaggcatact tcaaagactg tttgtttaaa
2581 gactgggagg agttggggga ggagattagg ttaaaggtct ttgtactagg aggctgtagg
2641 cataaattgg tctgcgcacc agcaccatgc aactttttca cctctgccta atcatctctt
2701 gttcatgtcc tactgttcaa gcctccaagc tgtgccttgg gtggctttgg ggcatggaca
2761 tcgaccctta taaagaattc ggagctactg tggagttact ctcgtttttg ccttctgact
2821 tctttccttc agtacgagat cttctagata ccgcaagctt gtctccctat agtgagtcgt
2881 attagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
2941 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
3001 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
3061 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
3121 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
3181 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
3241 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
3301 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
3361 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
3421 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
3481 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
3541 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
3601 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
3661 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
3721 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
3781 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
3841 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
3901 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
3961 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
4021 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
4081 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
4141 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
4201 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
4261 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
4321 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
4381 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
4441 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
4501 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
4561 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
4621 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
4681 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
4741 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
4801 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
4861 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
4921 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
4981 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
5041 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
5101 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
5161 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
5221 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
5281 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
5341 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
5401 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
5461 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
5521 aattggattt aggtgacact atagaatacg aattcgagct cgtattcaat ctaagcaggc
5581 tttcactttc tcgccaactt acaaggcctt tctgtgtaaa caatacctga acctttaccc
5641 cgttgcccgg caacggccag gtctgtgcca agtgtttgct gacgcaaccc ccactggctg
5701 gggcttggtc atgggccatc agcgcatgcg tggaaccttt tcggctcctc tgccgatcca
5761 tactgcggaa ctcctagccg cttgttttgc tcgcagcagg tctggagcaa acattatcgg
5821 gactgataac tctgttgtcc tatcccgcaa atatacatcg tttccatggc tgctaggctg
5881 tgctgccaac tggatcctgc gcgggacgtc ctttgtttac gtcccgtcgg cgctgaatcc
5941 tgcggacgac ccttctcggg gtcgcttggg actctctcgt ccccttctcc gtctgccgtt
6001 ccgaccgacc acggggcgca cctctcttta cgcggactcc ccgtctgtgc cttctcatct
6061 gccggaccgt gtgcacttcg cttcacctct gcacgtcgca tggagaccac cgtgaacgcc
6121 cactaaatat tgcccaaggt cttacataag aggactcttg gactctcagc aatgtcaacg
6181 accgaccttg aggcatactt caaagactgt ttgtttaaag actgggagga gttgggggag
6241 gagattaggt taaaggtctt tgtactagga ggctgtaggc ataaattggt ctgcgcacca
6301 gcaccatgca actttttcac ctctgcctaa tcatctcttg ttcatgtcct actgttcaag
6361 cctccaagct gtgccttggg tggctttggg gcatggacat cgacccttat aaagaatttg
6421 gagctactgt ggagttactc tcgtttttgc cttctgactt ctttccttca gtacgagatc
6481 ttctagatac cgcctcagct ctgtatcggg aagccttaga gtctcctgag cattgttcac
6541 ctcaccatac tgcactcagg caagcaattc tttgctgggg ggaactaatg actctagcta
6601 cctgggtggg tgttaatttg gaagatccag cgtctagaga cctagtagtc agttatgtca
6661 acactaatat gggcctaaag ttcaggcaac tcttgtggtt tcacatttct tgtctcactt
6721 ttggaagaga aacagttata gagtatttgg tgtctttcgg agtgtggatt cgcactcctc
6781 cagcttatag accaccaaat gcccctatcc tatcaacact t
//
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