LOCUS GLUP、RP3-495O10.2、HAK005771、PARK2CRG 3744 bp DNA circular SYN 26-AUG-2024
DEFINITION Gateway Donor vector containing OR2G6 , part of the Target
Accelerator Plasmid Collection..
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3744)
AUTHORS Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG,
Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
WC
TITLE Systematic Functional Interrogation of Rare Cancer Variants
Identifies Oncogenic Alleles.
JOURNAL Cancer Discov. 2016 Jul;6(7):714-26. doi:
10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
PUBMED 27147599
REFERENCE 2 (bases 1 to 3744)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 26, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date:
"2016-07"; volume: "6"; issue: "7"; pages: "714-26"
FEATURES Location/Qualifiers
source 1..3744
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 43..65
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 57..74
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 58..74
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 90..189
/gene="mutant version of attL"
/label=attL1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
protein_bind complement(1144..1243)
/gene="mutant version of attL"
/label=attL2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
primer_bind complement(1260..1279)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(1261..1279)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(1284..1300)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1284..1300)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
CDS 1731..2522
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 2615..3203
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 3104..3123
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 3357..3374
/label=L4440
/note="L4440 vector, forward primer"
terminator complement(3533..3560)
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator complement(3652..3738)
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
ORIGIN
1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
181 aagttggcac catggaggaa accaacaaca gctctgaaaa gggatttctt ctcctgggat
241 tttcagatca gcctcagcta gagaggtttc tttttgccat cattttgtac ttctacgtct
301 tgagccttct ggggaacact gccctcatac tagtatgttg tctggactcc agactccaca
361 ctccaatgta cttcttcctc agcaacctct cgtgtgtgga catctgcttt accaccagtg
421 ttgccccaca gttgctggtt accatgaata agaaagacaa aaccatgagc tacggtggct
481 gtgtggccca gctctacgtg gccatggggt tgggctcgtc tgagtgtatt ctcttggccg
541 tcatggctta tgaccgctat gctgctgtct gccggccact gcgctacata gccattatgc
601 accccaggtt ctgtgcgtct ctggccggtg gagcatggct cagcggcctc atcacctccc
661 taattcagtg ctccctcact gtgcagctgc ccctctgtgg tcatcgcaca ctggatcata
721 ttttctgtga ggtgccagtg ctcatcaaac tggcctgtgt ggatacgact ttcaacgagg
781 cagaactctt tgtggccagt gtagtctttc taattgtccc ggtgttactc atcttagtct
841 cctatggctt tatcactcaa gctgtgttaa ggataaaatc agctgcgggc cgccaaaagg
901 cctttgggac ctgttcgtct cacctggttg tggtcatcat tttctatggg accatcatat
961 tcatgtacct tcaaccggcc aataagagat ccaaaaacca gggaaagttt gtttctcttt
1021 tctataccat agtcacccca cttttaaacc ccattatcta cactctgaga aacaaagatg
1081 tgaaaggggc cttgaggacc ctgatactgg gtagtgctgc tggacaaagc cacaaggact
1141 gattgccaac tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt
1201 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc
1261 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc
1321 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagacca
1381 gccaggacag aaatgcctcg acttcgctgc tacccaaggt tgccgggtga cgcacaccgt
1441 ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
1501 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
1561 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
1621 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
1681 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatt atgagggaag
1741 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
1801 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
1861 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
1921 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
1981 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
2041 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
2101 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
2161 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
2221 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
2281 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
2341 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
2401 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
2461 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
2521 aaccctcgag ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag
2581 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
2641 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
2701 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
2761 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
2821 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
2881 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
2941 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
3001 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
3061 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
3121 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
3181 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
3241 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
3301 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
3361 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
3421 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
3481 gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc
3541 atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc
3601 ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct
3661 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg
3721 agcctttcgt tttatttgat gcct
//
