ATCC27064

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ATCC27064标准菌株基本信息

出品公司: ATCC
菌种名称: ATCC 27064, ATCC27064
菌种又名: [NRRL 3585]
菌株类型: Streptomyces clavuligerus,带小棒链霉菌
存储人: CE Higgens
分离来源: 土壤,南美
产品目录号: 27064
其他保藏库编号: AS 4.1611 =ATCC 27064 =BCRC 11518 =CBS 226.75 =DSM 40751 =KCTC 9095 =NBRC 13307
培养基:

ATCC Medium 196: Yeast malt extract agar 酵母麦芽汁琼脂
ATCC Medium 1877: ISP Medium 1 ISP培养基1号
 
生长条件: 30 ℃,  有氧
生物安全等级: 1
基因组序列号: ABJH00000000, CM000913, CM001015
基因组是否测序:
模式菌株:
应用: 细胞提取物能够通过环化作用将苯乙酰-L-半胱氨酰-D-缬氨酸转变为苄青霉素
菌株特点:
ATCC 27064是Streptomyces clavuligerus,带小棒链霉菌。

Nucleotide (GenBank) : A38835 Sequence 2 from Patent WO9412654.
 
Nucleotide (GenBank) : A39664 Sequence 1 from Patent WO9418326.
 
Nucleotide (GenBank) : A42941 Sequence 2 from Patent WO9503416.
 
Nucleotide (GenBank) : AJ000671 Streptomyces clavuligerus car and claR genes.
 
Nucleotide (GenBank) : AJ001743 Streptomyces clavuligerus pcd, cmcT and pbp2 genes.
 
Nucleotide (GenBank) : Z81324 S.clavuligerus ccaR and blp genes and open open reading frame.
 
Nucleotide (GenBank) : AF038457 Streptomyces clavuligerus strain ATCC 27064 plasmid pSCL2 terminus.
 
Nucleotide (GenBank) : L06213 Streptomyces clavuligerus clavaminate synthase 1 (cs1) gene, complete cds.
 
Nucleotide (GenBank) : Z49111 Streptomyces clavuligerus arginine biosynthesis cluster (argCJBDRGH genes).
 
Nucleotide (GenBank) : AJ238211 Streptomyces clavuligerus ORF1, encoding a protein involved in clavulanic acid biosynthesis.

 
参考文献:
Luengo JM, et al. Cyclization of phenylacetyl-L-cysteinyl-D-valine to benzylpenicillin using cell-free extracts of Streptomyces clavuligerus. J. Antibiot. 39: 1144-1147, 1986. PubMed: 3759664
 
Baldwin JE, et al. Isolation and partial characterization of ACV synthetase from Cephalosporium acremonium and Streptomyces clavuligerus. Evidence for the presence of phosphopantothenate in ACV synthetase. J. Antibiot. 44: 241-248, 1991. PubMed: 2010361
 
Zhang J, Demain AL. Regulation of ACV synthetase activity in the beta-lactam biosynthetic pathway of carbon sources and their metabolites. Arch. Microbiol. 158: 364-369, 1992.
 
Howarth TT, et al. Antibiotics. US Patent 4,525,352 dated Jun 25 1985
 
Perez-Llarena FJ, et al. A regulatory gene (ccaR) required for cephamycin and clavulanic acid production in Strptomyces clavuligerus: amplification results in overproduction of both beta-Lactam compounds. J. Bacteriol. 179: 2053-2059, 1997. PubMed: 9068654

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